Therefore, the lifelike fracture model made by 3Dp technology should be used as a means of premedical education. This randomized study revealed that the 3Dp model markedly improved the identification of complex spinal fracture anatomy by medical students and was equally appreciated and comprehended by both sexes. Pleasure, assistance, effect and confidence were more predominant in students in the 3Dp group than in those in the 3D and CT groups. Students in the 3Dp group were the first to answer all questions and there were no sex-related differences. Students in the 3Dp or 3D group performed significantly better than those in the CT group, although males in the 3D group scored higher than females. Altogether, 120 medical students (54.2% females) were randomized into three teaching module groups and asked to answer 10 key anatomical and 4 evaluative questions. Two spinal fracture simulation models were generated by 3Dp. Although the use of such lifelike models is beneficial, well-powered, randomized studies supporting this statement are scarce. Three-dimensional printing (3Dp) is being increasingly used in medical education.